With our new technique, you place DNA molecules at defined locations on a surface and each protein from a mixture will then self-assemble to its corresponding DNA sequence, like an automated paint-by-number kit.
Barber said: “Imagine you want to paint a picture on a canvas, but instead of painting in a normal fashion, you mix all of your paints together, splash it on the canvas, and the perfect picture emerges. Elledge’s laboratory.ĭescribing PICASSO, Dr. Karl Barber, is a 2018 Schmidt Science Fellow, with much of the work to develop the technology taking place during his Fellowship Research Placement in corresponding author Dr. Stephen Elledge at Harvard Medical School and Brigham and Women’s Hospital, Boston, has published the research online in Molecular Cell today (August 13, 2021). By then applying a blood sample to the PICASSO microarray, the proteins on the microchip that are recognized by patient antibodies can be identified. The researchers have called this technique ‘PICASSO’, short for peptide immobilization by Cas9-mediated self-organization. When these Cas9-fused proteins are applied to a microchip sporting thousands of unique DNA molecules, each protein within the mixture will self-assemble to the position on the chip containing its corresponding DNA sequence. The technology involves customizable collections of proteins which are attached to a variant of Cas9, the protein at the heart of CRISPR, that will bind to DNA but not cut it as it would when used for genetic modification.
Scientists have repurposed the genetic modification technology CRISPR to identify antibodies in patient blood samples in a move that could inspire a new class of medical diagnostics in addition to a host of other applications. Credit: Karl Barber, Schmidt Science Fellows Lead author, Karl Barber with a PICASSO microarray.
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